human pc3 metastatic pca cell line  (ATCC)

Journal: Cell Reports Methods

Article Title: Biobank of genetically defined murine prostate cancer tumoroids uncovers oncogenic pathways and drug vulnerabilities driven by PTEN-loss

doi: 10.1016/j.crmeth.2026.101370

Figure Lengend Snippet: The PDPK1/AKT/FLT DPI and tenovin-6 (T6) show high anti-cancer efficacy in murine tumoroids and human PCa cell lines (A) Dose-response curves for DPI (top) and T6 (bottom) for in vivo and in vitro Pten KO (left), Pten/Stat3 KO (middle), and Pten/Tp53 KO (right) tumoroids. Points represent means of technical duplicates per tumoroid line ( N = 3). Curve fitting was performed using GraphPad Prism 8.0.2. (B) Bar graphs showing means and ±SD of half-maximal inhibitory concentration (IC50) for DPI (top) and T6 (bottom) for in vivo and in vitro tumoroid lines of all genotypes ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA, Tukey’s test). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05. (C) Bar graphs depicting means and ±SD of IC50 values of DPI (left) and T6 (right) on human PCa cell lines. 22RV1: primary PCa; LNCaP: metastatic PCa; DU145, PC3: metastatic castration-resistant PCa ( N = 3). Statistical analysis was performed using GraphPad Prism 8.0.2 (one-way ANOVA). p > 0.05 if not specified otherwise, ∗ p ≤ 0.05; ∗∗ p ≤ 0.01. (D) Heatmaps of synergy scores calculated with the highest single agent (HSA) model for DPI and enzalutamide (left), and T6 and enzalutamide (right) on the human LNCaP cell line. Values > 0 represent synergistic effects, and values < 0 represent antagonistic effects. IC50 concentrations of respective compounds are underlined ( N = 3). See also .

Article Snippet: Human PC3 metastatic PCa cell line , ATCC , CRL-1435; RRID:CVCL_0035.

Techniques: In Vivo, In Vitro, Concentration Assay

Journal: Science advances

Article Title: miR-26a regulates extracellular vesicle secretion from prostate cancer cells via targeting SHC4, PFDN4, and CHORDC1.

doi: 10.1126/sciadv.aay3051

Figure Lengend Snippet: Fig. 2. SHC4, PFDN4, and CHORDC1 are involved in miR-26a–mediated EV secretion. (A) Venn diagram of predicted miR-26a targets (TargetScan) and transcripts that were experimentally repressed >2-fold by miR-26a overexpression in PCa cells (PC3M or PC3) relative to control conditions. (B) Schematic of the high-throughput com- patible EV biogenesis assay to choose EV biogenesis–regulating genes. (C) Venn diagram showing genes that suppress EV secretion evaluated by ExoScreen. The genes whose relative EV secretion/cell viability was lower than that of miR-26a plus 0.3 were selected in each assay. The secretion of EV was evaluated by ExoScreen, and the cell viability was measured through the MTS assay. (D) Effect of siRNAs against candidate genes on EV secretion in PC3M cells. The EV secretion per cell was evaluated by the signal intensity of ExoScreen per cell. The values are depicted as the fold change relative to the negative control siRNA (control). The values are the means ± SE (n = 3). *P < 0.05; **P < 0.01; and n.s., not significant. (E) Effect of siRNAs against candidate genes on EV secretion per PC3M cell. The particle number of EVs was measured using a nanoparticle tracking system. The values are the means ± SE (n = 3). *P < 0.05; n.s., not significant. (F) Effect of SHC4, PFDN4, and CHORDC1 siRNA on the mRNA expres- sion level of each gene. -Actin was used as an internal control. Error bars represent the SE deduced by Student’s t test (*P < 0.05 and **P < 0.01). n.s., no significant differ- ence. The data are representative of at least three independent experiments. The values are the means ± SE (n = 3). **P < 0.01.

Article Snippet: The human PCa epithelial metastatic cell line PC3 (ATCC CRL-1435) was purchased from the American Type Culture Collection (ATCC).

Techniques: Over Expression, Control, High Throughput Screening Assay, MTS Assay, Negative Control

Journal: Cancer Science

Article Title: The miR‐1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer

doi: 10.1111/cas.14535

Figure Lengend Snippet: Screening of miRNAs regulating extracellular vesicle secretion. A, Amount of extracellular vesicle (EV) secretion in 22Rv1, PC3, and PC3M cell. The number of EVs was examined using a nanoparticle tracking system. Values represent mean ± SE (n = 3). The statistical significance was calculated using Dunnett's t test (** P < .01). B, Representative image of EVs isolated from 22Rv1 and PC3 cells under transmission electron microscopy. Scale bars, 100 nm. C, Result of primary screening. Distribution of the score for each sample calculated from the amount of EVs and cell viability; 1728 miRNAs are plotted as red dots or gray dots according to cut‐off value (score < 0.15). D, Result of the secondary screening. Distribution of the scores for 33 selected samples. Thirty‐three miRNAs are plotted according to cut‐off value (score < 0.15). E, Effect of miR‐518, miR‐1253, miR‐1908, and miR‐4460 on the EV secretion per 22Rv1 cell. Number of EV secreted from each 22Rv1 cell was examined using a nanoparticle tracking system. Values represent mean ± SE (n = 3). The statistical significance was calculated using Dunnett's t test (* P < .05, ** P < .01)

Article Snippet: Human metastatic PCa cell lines PC3 (ATCC CRL‐1435) and PC‐3M‐luc‐C6 (PC3M) (Xenogen) were cultured in RPMI 1640 medium (Thermo Fisher Scientific) supplemented with 10% heat‐inactivated FBS and 1% AA.

Techniques: Isolation, Transmission Assay, Electron Microscopy